![]() ![]() When Fang is talking, or if I'm roleplaying, the lettering will be small like this. ![]() ((By the way, when you see these marks ")), ((" at the beginning and ending of a sentence, that means me, the admin of this account is talking. But, I just randomly talk to people, nowadays, and make statuses. I used to have a blog, but I've been to busy, or lazy to actually sit down and write it lately. I like to talk to people, and I do have a facebook account! So, you can also contact me on there, if you would like. I'm also known as a "bird freak." Or that's what Max refers us, too, anyway. I guess I could say I'm 15 years old, but that's just a number of years that I've been alive. <3ĪLSO I'm using Avan Jogia as a face claim for his profile pictures on Facebook, and here. If you haven't read that series, OMG, you should go read it, because it's an amazing story. The ToF-SIMS images of Cl − and PO 3 − were acquired at m/ z 35 and 79, respectively.(( Yo, Maximum Ride fans, and random deviants who come upon this profile! <3 This is more like a Role Play account of the character Fang from " Maximum Ride" by James Patterson. (N) and (O) Merged ToF-SIMS images of Cl − (red) and PO 3 − (green) and the CLSM image of EYFP-HMGB1 (blue) or DAPI (blue). (L) and (M) ToF-SIMS images of: (L) Cl − and (M) PO 3 − using 3000 BIB scans. (J) Merged image of the ToF-SIMS images of the total ions, Cl − and PO 3 −. (G)–(I) ToF-SIMS images of: (G) total ions (H) Cl − and (I) PO 3 − using 100 BIB scans. (E) Merged image of the CLSM images of EYFP-HMGB1 (green), DAPI (blue) and ToF-SIMS image of the total ions (red). (D) ToF-SIMS image of the total ions using 10 BIB scans. (B) and (C) CLSM images of (B) EYFP-HMGB1 and (C) the DAPI stained nucleus. ![]() (A), (F) and (K) Bright field images of a single cell framed in a red box: (A) before (F) after 10 cycles of GCIB sputtering on (A) and (K) after 100 BIB scans on (F). COSIMSi of lyophilized HeLa cells without treatment using cisplatin. This journal is © The Royal Society of Chemistry.įig. This finding suggests that Smad3 and its related signalling pathway are most likely involved in the intracellular response to cisplatin induced DNA damage. More significantly, for the first time, similar integrated imaging revealed that the cisplatin lesions at Smad-binding elements, for example GGC(GC)/(CG) and AGAC, disrupted the interactions of Smad3 with DNA, which was evidenced by the remarkable reduction in the expression of Smad-specific luciferase reporters subjected to cisplatin treatment. The superposition of the fluorescence and the mass spectrometry (MS) signals indicate the formation of HMGB1-Pt-DNA ternary complexes in the cells. We utilized confocal microscopy imaging to map EYFP-fused HMGB1 and fluorescent dye-stained DNA in single cells, followed by the visualization of cisplatin using high spatial resolution (200-350 nm) time of flight secondary ion mass spectrometry (ToF-SIMS) imaging of the same cells. Herein, we developed a dual-modal microscopy imaging strategy to investigate, in situ, the formation of ternary binding complexes of the transcription cofactor HMGB1 and transcription factor Smad3 with cisplatin crosslinked DNA in single cells. However, the way in which cisplatin-induced DNA lesions regulate interactions between transcription factors/cofactors and genomic DNA remains unclear.
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